ex vivo gene transfer meaning in English

先体外后体内基因转移

3 . using clamp technique , ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig
供肝冷保存时，采用血管夹技术lamptechnique ）经门静脉灌注携带融合基因hllctla4dg的重组腺病毒，于术后3天、 7天能定性检测到hllctla4ig在受体外划血卜1 。
Using ex vivo gene transfer technique , exogenous gene was introduced to the liver graft during cold preservation and express locally in the graft . the effect of inhibition of rejection and inducing liver graft tolerance was observed . through this study , the possibility of achieving graft tolerance by gene transfer without routine immunosuppressive drugs was explored
我们采用adeasy腺病毒载体系统，自行构建携带huctla4 - ig的重组腺病毒，通过先体外后体内( exvivo )基因转移技术，于供肝冷保存时，将该治疗基因导入大鼠移植肝，使其于移植肝局部表达，观察其抑制排斥反应，诱导大鼠移植肝免疫耐受的作用。